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目的 探讨检测红细胞表面抗体IgG对新生儿母子血型不合溶血病的诊断意义。 方法 选自 2 0 0 1年 11月～ 2 0 0 2年 7月间收治的足月儿新生儿溶血病 5 0例 ,根据子直接Coombs试验结果分为两组 :阳性组 38例 ,阴性组 12例。同期住院的无黄疸、无母子血型不合的 30例患儿作为对照组。利用流式细胞术和抗人IgG单克隆抗体检测红细胞表面抗体IgG。 结果 IgG测定的均值在子直接Coombs试验阳性组为 (12 .6 4± 16 83) % ,阴性组为 (4 .5 4± 2 5 4 ) % ,对照组为 (1.5 9±0 79) % ,三组间差异有非常显著性 (P <0 .0 1)。在总胆红素 >4 2 7.5 μmol/L时 ,随总胆红素的升高 ,IgG与总胆红素的直线相关性增加 (r =0 .6 78)。 结论 检测新生儿溶血病患儿红细胞表面抗体IgG可辅助诊断新生儿母子血型不合溶血病 ,尤其是子直接Coombs试验阴性时。
Objective To investigate the diagnostic significance of detecting erythrocyte surface antibody (IgG) on hemolytic hemolytic disease in newborns. Methods Fifty children with term neonates with hemolytic disease of full-term infants admitted from November 2001 to July 2002 were divided into two groups according to the results of sub-direct Coombs test: 38 in the positive group and negative in the negative group 12 cases. In the same period, there was no jaundice in the hospital and 30 children without maternal and child blood group incompatibility as the control group. Detection of erythrocyte surface antibody IgG by flow cytometry and anti-human IgG monoclonal antibody. Results The mean value of IgG assay was (14.6 ± 16.83)% in the sub-direct Coombs test, (4.54 ± 2.54)% in the negative group and (1.59 ± 0.79)% in the control group , The difference between the three groups was significant (P <0. 01). With total bilirubin> 42 2 μmol / L, the linear correlation between IgG and total bilirubin increased with total bilirubin (r = 0.788). Conclusion Detection of erythrocyte surface antibody (IgG) in children with hemolytic disease can be used to diagnose neonatal maternal hemopoietic incompatibility hemolytic disease, especially when sub-direct Coombs test is negative.