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目的：观察３ 种细胞因子对体外培养大鼠肝细胞产生一氧化氮（ ＮＯ） 的影响及ＮＯ 在实验性肝损伤中的作用。方法与结果：给大鼠注射内毒素后分离肝细胞，培养液中加入细胞因子（γ－ ＩＦＮ，ＩＬ－ ２ ，ＴＮＦ－ α） ，上清液中ＮＯ 含量增加，两种以上细胞因子联合应用，ＮＯ 含量增加更明显；大鼠注射内毒素及Ｌ－ 精氨酸，血清中ＮＯ 含量增加，肝损伤减轻；大鼠给硫代乙酰胺（ＴＡＡ） 灌胃，并注射ＮＯ 生成抑制剂Ｌ－ ＮＮＡ，动物出现嗜睡及肝昏迷脑电图，血浆ＬＤＨ、Ｃｒ升高。结论：内毒素及细胞因子促进ＮＯ 合成，ＮＯ 在内毒素诱发的肝损伤及ＴＡＡ 致肝功能不全中具有对抗作用。
Objective: To observe the effects of three cytokines on the production of nitric oxide (NO) in cultured rat hepatocytes and the role of NO in experimental liver injury. Methods and Results: Hepatic cells were isolated from rats after injection of endotoxin, cytokines (γ-IFN, IL-2 and TNF-α) were added to the culture medium, and the content of NO in the supernatant was increased. Two or more cytokines were used in combination , The content of NO increased more obviously; the endotoxin and L-arginine were injected into the rats, the content of NO in the serum increased and the liver injury alleviated. The rats were given intragastric administration of TAA and NO- NNA, animals appear lethargy and hepatic coma EEG, plasma LDH, Cr increased. CONCLUSION: Endotoxin and cytokines promote NO synthesis, NO antagonizes endotoxin-induced hepatic injury and TAA-induced hepatic dysfunction.