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[目的]观察华蟾素对白血病细胞的凋亡诱导作用及其与三氧化二砷(As2O3)的协同作用。[方法]通过形态学方法、DNA电泳、AnnexinV标记法检测华蟾素或(和)As2O3 作用后HL_60细胞的凋亡发生情况。[结果]0.0625μg/ml～0.5μg/ml的华蟾素作用48h后 ,HL_60细胞发生凋亡 ,并具有一定的量效关系 ;华蟾素与As2O3 合用后对HL_60细胞的凋亡诱导作用明显增强。[结论]华蟾素对白血病细胞具有凋亡诱导作用 ,并且与As2O3 具有协同作用。
[Objective] To investigate the apoptosis-inducing effect of cinobufacini on leukemia cells and its synergistic effect with arsenic trioxide (As2O3). [Method] Morphological methods, DNA electrophoresis and Annexin V labeling were used to detect the apoptosis of HL60 cells after treated with cinobufacini or (and) As2O3. [Result] After treated with 0.0625μg / ml ~ 0.5μg / ml of cinobufacine for 48h, the apoptosis of HL-60 cells was observed and had some dose-effect relationship. The combination of cinobufotalin and As2O3 significantly induced the apoptosis of HL-60 cells Enhanced. [Conclusion] Cinobufacini has apoptosis-inducing effect on leukemia cells and has a synergistic effect with As2O3.