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通过比较血清HBeAg、HBV DNA与其它血清标志间的不同相关程度,我们探讨了几项血清学检查用于献血员筛选的相对可靠性。方法和结果用上海医化所ELISA试剂检出HBeAg阳性个体686人。同时用上海医化所ELISA试剂查抗HBc 1 :10~3),用北京生物制品所RPHA试剂查HBsAg(1:8)。HBeAg阳性者血清抗HBc阳性率95.8%,显著高于HBsAg阳性率91.8%,P<0.02。用北京医学院肝病研究所~(32)P标记HBVDNA探针通过斑点杂交试验测123名肝炎病人的血清,同时按前述方法查HBeAg、HBsAg、抗HBc,用北京解放军302医院抗
By comparing the different degrees of correlation between serum HBeAg, HBV DNA and other serum markers, we explored the relative reliability of several serological tests for blood donor screening. Methods and Results 686 HBeAg-positive individuals were detected by ELISA from Shanghai Medical Institute. At the same time with the Shanghai Medical Institute ELISA reagent anti-HBc 1: 10 ~ 3), with Beijing Institute of Biological reagents RPHA reagent check HBsAg (1: 8). The positive rate of anti-HBc in serum of HBeAg-positive patients was 95.8%, which was significantly higher than that of HBsAg-positive patients (91.8%, P <0.02). The serum of 123 hepatitis patients was tested by dot blot hybridization with HBVDNA probe labeled with P (32) P of Hepatology Institute of Beijing Medical College. HBeAg, HBsAg and anti-HBc were also detected by the above method.