论文部分内容阅读
目的:探讨喉癌组织中HMGA2的作用及其临床意义。方法:采用实时荧光定量PCR(RQ-PCR)检测HM-GA2基因在20例喉癌组织和癌旁正常黏膜上皮组织中mRNA的水平,同时制作组织芯片应用免疫组化检测其在50例喉癌组织和癌旁正常黏膜上皮组织中蛋白水平的表达。结果:喉癌组织和癌旁正常黏膜上皮组织HMGA2mRNA的相对表达量分别为0.358±0.261和0.203±0.151,HMGA2蛋白的阳性率分别为86.0%(43/50)和27.3%(12/44),两组比较差异有统计学意义,P值均<0.05;HMGA2的表达与与患者性别、年龄无显著相关性,P>0.05;与TNM分期和淋巴结转移有显著相关性,P<0.05。结论:HMGA2在喉癌组织中的表达与肿瘤的发生、侵袭和转移密切相关,可能成为喉癌新的肿瘤标志或预后因子。
Objective: To investigate the role of HMGA2 in laryngeal carcinoma and its clinical significance. Methods: The mRNA levels of HM-GA2 gene in 20 cases of laryngeal carcinoma and adjacent normal mucosa epithelial tissues were detected by real-time fluorescence quantitative PCR (RQ-PCR). At the same time, immunohistochemistry was used to detect the mRNA level of HM- Tissue and adjacent normal mucosa epithelial tissue protein expression. Results: The relative expression levels of HMGA2 mRNA in laryngeal carcinoma tissues and adjacent normal mucosa epithelial tissues were 0.358 ± 0.261 and 0.203 ± 0.151, respectively. The positive rates of HMGA2 protein were 86.0% (43/50) and 27.3% (12/44), respectively. The difference between the two groups was statistically significant (P <0.05). The expression of HMGA2 had no significant correlation with the sex and age of patients (P> 0.05). There was a significant correlation between the expression of HMGA2 and TNM stage and lymph node metastasis (P <0.05). Conclusion: The expression of HMGA2 in laryngeal carcinoma is closely related to tumorigenesis, invasion and metastasis, which may be a new tumor marker or prognostic factor for laryngeal cancer.