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目的:观察GluR1在骨癌痛(bone cancer pain,BCP)模型小鼠中央杏仁核中的表达变化。方法:健康C57小鼠分为假手术对照组(n=100)和骨癌痛组(n=100),每组均在术前和术后7、14、21、28 d先进行行为学检测,检测完毕后取材,进行免疫组化染色和Western Blot检测,观察GluR1在中央杏仁核中的表达变化。结果:从癌细胞接种后第7 d开始,BCP组出现自发缩足次数增多、PWT值降低,14 d后与Sham组比较开始有明显差异,表明模型建立成功;免疫组织化学染色显示GluR1在正常C57小鼠中央杏仁核中的表达水平较低,但在建模术后小鼠中央杏仁核中GluR1的表达开始逐渐升高,图像分析表明GluR1的光密度与对照组比较,第14 d时差异有统计学意义(P<0.05),BCP组21 d时GluR1的表达达高峰(P<0.01),Western Blot检测结果亦与之相符。结论:骨癌痛小鼠GluR1可能在杏仁核参与神经病理性痛的过程中具有重要作用。
Objective: To observe the expression of GluR1 in the central amygdala of mice with bone cancer pain (BCP). Methods: Healthy C57 mice were divided into sham-operation control group (n = 100) and bone cancer pain group (n = 100), and each group was subjected to behavioral tests before operation and at 7, 14, , After the test was taken, immunohistochemical staining and Western Blot detection, observed GluR1 in the central amygdala in the expression changes. Results: From the 7th day after inoculation of cancer cells, the number of spontaneous contraction increased and the PWT decreased in BCP group, and the difference was significant at the 14th day after the inoculation, indicating that the model was established successfully. Immunohistochemical staining showed that GluR1 was in normal The expression of GluR1 in central amygdaloid nucleus of C57 mice began to decrease after modeling, and the optical density of GluR1 in control group was significantly lower than that in control group (P <0.05). The expression of GluR1 reached its peak at 21 d in BCP group (P <0.01), and the result of Western Blot was also consistent with it. Conclusion: GluR1 may play an important role in the process of amygdala participating in neuropathic pain.