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目的研究氟对体外培养大鼠睾丸支持细胞雄激素结合蛋白(ABP)和抑制素B(INHB)转录水平的影响。方法建立支持细胞体外培养模型,以浓度分别为2.5、5.0、10.0和20.0mg/L氟化钠溶液染毒细胞,用RT-PCR方法检测ABP和INHB mRNA的相对表达量。结果①ABP mRNA相对表达量在各剂量组与对照组相比,2.5mg/L组高于对照组,且差异有统计学意义(P<0.05);5.0mg/L高于对照组,但差异没有统计学意义;其余各组低于对照组,但差异没有统计学意义(P>0.05)。②INHB mRNA相对表达量在各剂量组与对照组比较,2.5和5mg/L组均高于对照组,差异具有统计学意义(P<0.05);其余两组低于对照组,但是差异均无统计学意义(P>0.05)。结论在2.5～20.0mg/L剂量范围内,未观察到氟对体外培养大鼠睾丸支持细胞ABP和INHB mRNA的表达有明显影响。
Objective To investigate the effect of fluoride on the transcriptional level of androgen-binding protein (ABP) and inhibin B (INHB) in cultured rat testicular sertoli cells. Methods Support cells were cultured in vitro, and the cells were exposed to sodium fluoride at concentrations of 2.5, 5.0, 10.0 and 20.0 mg / L, respectively. The relative expression of ABP and INHB mRNA was detected by RT-PCR. Results ①ABP mRNA expression in each dose group compared with the control group, 2.5mg / L group was higher than the control group, and the difference was statistically significant (P <0.05); 5.0mg / L higher than the control group, but no difference Statistical significance; The other groups were lower than the control group, but the difference was not statistically significant (P> 0.05). ② The relative expression of INHB mRNA in each dose group compared with the control group, 2.5 and 5mg / L group were higher than the control group, the difference was statistically significant (P <0.05); the other two groups were lower than the control group, but the difference was not statistically Significance (P> 0.05). Conclusion In the dose range of 2.5-20.0 mg / L, no significant effect of fluoride on the expression of ABP and INHB mRNA in cultured rat testis supportive cells was observed.