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以[~3H]地塞米松(Dex)为配体,建立了狗外周血白细胞糖皮质激素受体(GCR)的放射配体结合测定方法,Scatchard作图分析[~3H]Dex特异结合呈一直线,表观结合容量(R_o)和平衡解离常数(Kd)分别为7.30±1.98fmol/10~6个细胞和4.69±2.65_nM((?)±SD,N=5),特异结合有明显的甾体特异性。用饱和浓度的[~3H]Dex测定了22只无应激条件下成年健康狗外周血白细胞的[~3H]Dex特异结合,结果为3745±436位点/细胞((?)±SD),雄雌之间差异不显著。本方法具有取材方便,重复性好及用血量少等优点,适用于一般实验室。
Radioligand binding assay of dog peripheral blood leukocyte glucocorticoid receptor (GCR) was established using [~ 3H] dexamethasone (Dex) as a ligand. Scatchard mapping showed that [~ 3H] Dex specific binding was consistently observed The apparent binding capacity (R_o) and equilibrium dissociation constant (Kd) were 7.30 ± 1.98 fmol / 10 ~ 6 cells and 4.69 ± 2.65 nM (± SD ± SD, N = 5) Steroidal specificity. [~ 3H] Dex specific binding of peripheral blood leukocytes in healthy healthy dogs was measured at 3745 ± 436 sites / cell (± SD) with saturated concentrations of [~ 3H] Dex, The difference between male and female is not significant. The method has the advantages of convenient drawing, good repeatability, less blood supply and the like, and is suitable for general laboratories.