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For a rotational relativistic Birkhoffian system, a set of the Lie symmetries and conservation laws is given under infinitesimal transformations. On the basis of the invariance of rotational relativistic Birkhoffian equations under infinitesimal transformations, Lie symmetrical transformations of the system are constructed, which only depend on the Birkhoffian variables. The determining equations of Lie symmetries are given, and a new type of non-noether conserved quantities are directly obtained from Lie symmetries of the system. An example is given to illustrate the application of the results.