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It is well known that the quantum fluctuation of entanglement (QFE) between Unruh-De Witt detector (modeled by a two-level atom) is always investigated in a relativistic setting. However, both of the Unruh radiation and quantum fluctuation effects play an important role in precise measurements of quantum entanglement. In this paper, we have quantitatively analyzed how the relativistic motion affects the QFE for two entangled Unruh-De Witt detectors, one of which is accelerated and interacting with the neighbor exteal scalar field. Our results show that the QFE, which initially increases by the Unruh thermal noise, will suddenly decay when the acceleration reaches to a considerably large value. Therefore, the relativistic effect will lead to non-negligible QFE effect. We also find that the initial QFE (without acceleration effect) reaches its minimum value at the maximally entangled state and the separable state. More importantly, our analysis demonstrates that although the QFE has a huge decay when the acceleration is greater than～0.96, the ratio of?E/C is still very large, due to the simultaneous decay of concurrence to a very low value. Finally, enlightened by the well-known equivalence principle, we discuss the possibility of applying the above findings to the dynamics of QFE under the influence of gravitation field.