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The interactions of carbofuran and DNA were studied using voltammetry and fluorescence spectroscopy.The formation of carbofuran-DNA makes the current peak of DNA decreased by voltammetry method.The binding number(n) and constant(K_a) for complex carbofuran-DNA were calculated to be 1.06±0.04 and 0.11±0.03mol~(-1) L,respectively by fluorescence measurement.Chemometrics approach,such as singular value decomposition(SVD) was used to evaluate the number of spectral species in the drug-DNA binding process.And the pure spectra and concentration profiles in the kinetic system were clearly deduced by multivariate curve resolution alternating least squares(MCR-ALS) with the initial estimates by evolving factor analysis(EFA).
The interactions of carbofuran and DNA were studied using voltammetry and fluorescence spectroscopy. The formation of carbofuran-DNA makes the current peak of DNA decreased by voltammetry method. The binding number (n) and constant (K_a) for complex carbofuran-DNA were calculated to respectively 1.06 ± 0.04 and 0.11 ± 0.03 mol ~ (-1) L, respectively by fluorescence measurement. Chemometrics approach, such as singular value decomposition (SVD) was used to evaluate the number of spectral species in the drug-DNA binding process. And the pure spectra and concentration profiles in the kinetic system were clearly deduced by multivariate curve resolution alternating least squares (MCR-ALS) with the initial estimates by evolving factor analysis (EFA).