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目的探讨锌对胰岛素抵抗靶细胞活力和葡萄糖消耗量的影响。方法建成Hep G2细胞、3T3-L1细胞和L6成肌细胞胰岛素抵抗模型后,用10-7mol/L胰岛素和不同浓度锌(0、20、50、100、200、400μmol/L)孵育细胞,通过MTT法和葡萄糖氧化酶法测定基础状态和胰岛素刺激状态下3种胰岛素抵抗细胞葡萄糖的消耗量。结果不同剂量的锌孵育3种细胞3 h后,200、400μmol/L的锌可明显抑制各组细胞的活力(P<0.05);50~100μmol/L的锌干预3 h能在不同程度上提高Hep G2、3T3-L1正常细胞和胰岛素抵抗细胞的葡萄糖消耗量,但其作用效果明显低于胰岛素;其中100μmol/L的锌与胰岛素共同作用的效果分别优于胰岛素或锌单独作用的效果(P<0.05);对于L6肌细胞,20、50和100μmol/L锌均能明显提高基础状态下正常细胞和胰岛素抵抗细胞的葡萄糖消耗量(P<0.05);仅有20μmol/L锌和胰岛素共同作用的效果优于胰岛素或锌单独作用的效果(P<0.05)。结论不同浓度的锌可以在一定程度上提高3种胰岛素抵抗的靶细胞葡萄糖摄取量,且不同的细胞具有不同的适宜作用剂量。
Objective To investigate the effect of zinc on the target cell viability and glucose consumption in insulin resistance. Methods Hep G2 cells, 3T3-L1 cells and L6 myoblasts insulin resistance model were established. After the cells were incubated with 10-7mol / L insulin and different concentrations of zinc (0, 20, 50, 100, 200 and 400μmol / L) MTT and glucose oxidase method to determine the basal state and insulin stimulated glucose consumption of three kinds of insulin resistance cells. Results After incubated with different doses of zinc for 3 h, zinc of 200 and 400 μmol / L significantly inhibited the viability of all the three groups (P <0.05), and the levels of zinc increased from 50 μmol / L to 100 μmol / L for 3 h Hep G2, 3T3-L1 normal cells and insulin resistance cells, but its effect was obviously lower than that of insulin. The combined effect of 100μmol / L zinc and insulin was better than that of insulin or zinc alone (P <0.05). For L6 myocytes, 20, 50 and 100μmol / L zinc significantly increased the glucose consumption of normal cells and insulin resistance cells (P <0.05). Only 20μmol / L zinc interacted with insulin The effect is better than the effect of insulin or zinc alone (P <0.05). Conclusion Different concentrations of zinc can improve the glucose uptake of target cells in three kinds of insulin resistance to a certain extent, and different cells have different suitable dose of action.