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通过对甘蔗渣进行不同的预处理,确定了提高纤维素酶糖化率和酱油曲霉发酵液絮凝率的方法。利用正交实验对固液比、颗粒粒径、溶剂浓度和预处理时间及其交互作用进行考察,确定较优的工艺方案。结果表明,在相同条件下,碱处理能够有效提高纤维素酶的糖化率,处理后甘蔗渣的酶解液中还原性糖浓度和酱油曲霉发酵液的絮凝率分别为3.46 g/L和97.82%。碱处理的最优方案为固液比1∶20,颗粒粒径小于0.95 mm,1%NaOH处理60 min,利用该方案得到的还原性糖浓度和絮凝率分别为4.38 g/L和98.94%。不同批次的甘蔗渣经该方法处理后,实验结果的差异性不大,显示该方案具有较好的稳定性。
Through different pretreatment of bagasse, the methods to improve the saccharification rate of cellulase and the flocculation rate of Aspergillus sojae fermentation broth were determined. Orthogonal experiments were used to investigate the solid-liquid ratio, particle size, solvent concentration and pretreatment time and their interaction, and to determine the optimal process scheme. The results showed that under the same conditions, alkali treatment could effectively improve the saccharification rate of the cellulase, the reducing sugar concentration of the enzymatic hydrolyzate of the treated bagasse and the flocculation rate of Aspergillus sojae fermentation broth were 3.46 g / L and 97.82% . The optimum conditions for alkali treatment were solid-liquid ratio 1:20, particle size less than 0.95 mm and NaOH treatment 60 min. The reducing sugar concentration and flocculation rate were 4.38 g / L and 98.94% respectively. Different batches of bagasse treated by this method, the experimental results of the difference is not large, shows that the program has good stability.