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应用酶联免疫检测技术 ,检测沈阳地区 390 2例不同人群血清的抗 - HCV,并应用引物特异 PCR法对其中 10 0例血清标本进行 HCV基因分型。结果 :正常人群抗 - HCV阳性率为 0 .4 2 %～ 1.66% ;输血后及散发性肝炎 (除外 HAV、HBV、EBV、CMV感染 )、乙型肝炎、肝炎后肝硬化 (除外 HBV感染 )、原发性肝癌等组抗 - HCV阳性率显著高于无输血史的非肝病病人组 ( P<0 .0 1) ;10 0例血清标本中 HCV- 型感染占 58%、HCV- 型 2 7%、 / 混合型 14%、未分型 1% ,正常人群HCV感染者中 HCV基因型以 型为主 ( 80 % ) ,丙型肝炎后肝硬化病人 HCV基因型以 型为主( 91.7% )。提示 :各类肝病病人是 HCV感染的高危人群 ;沈阳地区 HCV感染以 型为主 ,其次为 型及 / 混合型 ,HCV基因型可能与丙型肝炎病情轻重有关。
The serum anti-HCV of 390 people in Shenyang was tested by enzyme-linked immunosorbent assay (ELISA). HCV genotypes of 100 serum samples were detected by primer-specific PCR. Results: The positive rates of anti - HCV in normal population ranged from 42.2% to 1.66%. After transfusion and sporadic hepatitis (except HAV, HBV, EBV and CMV infection), hepatitis B and cirrhosis after hepatitis (except HBV infection) , The positive rate of anti-HCV in primary liver cancer and other groups was significantly higher than that in non-liver disease patients without transfusion history (P <0.01); HCV-type infection was 58% in 100 serum samples and HCV-type 2 HCV genotype was predominant in 80% of HCV infected subjects and HCV genotype was predominant in patients with hepatitis C cirrhosis (91.7% ). Hint: All kinds of liver disease patients are at high risk of HCV infection; HCV infection in Shenyang is mainly type, followed by type and / or mixed type, HCV genotype may be related to the severity of hepatitis C disease.