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目的 探讨大鼠胰岛B细胞功能对Th1/Th2淋巴细胞分化的影响及其机制。方法 2 0 0 2 - 11～ 2 0 0 3- 0 9在北京大学人民医院将 4 0只雄性SD大鼠随机分A、B、C、D和E组 ,每组 8只。A组 :腹腔注射链脲菌素(STZ)建成 1型糖尿病模型 ;B组 :腹腔注射卵清蛋白 (OVA )致敏 +OVA激发建成哮喘大鼠模型 ;C组 :腹腔注射STZ +OVA致敏和激发 ;D组 :腹腔注射STZ +皮下注射胰岛素 +OVA致敏和激发 ;E组为对照组。检测各组血清C肽浓度、支气管肺泡灌洗液 (BALF)IL 4和IFN γ质量浓度。结果 与对照组比较 ,A、C组的血清C肽浓度显著下降 ,而BALF的IFN γ质量浓度显著增高 ,IL 4质量浓度显著下降 ;与C组比较 ,D组经注射胰岛素后 ,其BALF中的IFN γ质量浓度显著降低 (137 6 3± 9 94vs 111 0 0± 12 14 ,P <0 0 5 ) ,IL 4质量浓度显著增高 (11 0 0± 3 93vs15 6 3± 2 6 7,P <0 0 5 )。结论 大鼠胰岛B细胞功能对Th1/Th2淋巴细胞的分化有调节作用。
Objective To investigate the effect of rat pancreatic islet B cell function on Th1 / Th2 lymphocyte differentiation and its mechanism. Methods Two hundred and twenty male SD rats were randomized into groups A, B, C, D and E in Peking University People ’s Hospital at Peking University People’ s Hospital. Group A: model of diabetes mellitus (STZ) was established by intraperitoneal injection of streptozotocin (STZ); model of asthma was established by sensitization of OVA + OVA induced by intraperitoneal injection; group C: sensitization by STZ + OVA by intraperitoneal injection of STZ + Excitation; Group D: intraperitoneal injection of STZ + subcutaneous injection of insulin + OVA sensitization and challenge; E group as the control group. The concentrations of serum C-peptide and IL-4 and IFN-γ in bronchoalveolar lavage fluid (BALF) were detected. Results Compared with the control group, the concentration of serum C-peptide in group A and C decreased significantly, while the concentration of IFN-γ in BALF increased significantly and the concentration of IL-4 decreased significantly. Compared with group C, the insulin concentration in BALF (137 6 3 ± 9 94 vs 111 0 0 ± 12 14, P <0 05), and the concentration of IL 4 was significantly increased (11 0 0 ± 3 93 vs 15 6 3 ± 2 6 7, P < 0 0 5). Conclusion The function of islet B cells in rats may play a regulatory role in the differentiation of Th1 / Th2 lymphocytes.