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土壤细菌群落在蔬菜栽培中发挥着重要作用。基于DNA和RNA水平,利用PCR-DGGE技术研究了不同栽培环境下有机与常规蔬菜土壤细菌群落多样性差异,以及土壤理化性质与细菌群落多样性的关系。结果表明:不同栽培方式下土壤细菌多样性存在明显差异,土壤微生物的优势种群和数量受有机、常规栽培和季节影响,有机栽培较之常规栽培能够显著增加土壤细菌群落多样性;聚类分析表明,16S rDNA细菌群落多样性与季节相关,而16S rRNA细菌群落多样性与栽培方式相关;差异条带测序显示,大多细菌与不可培养细菌种属有较高同源性,其余9种推测属于假单胞菌属;CCA分析说明pH是影响土壤细菌群落多样性的主要因素,有机栽培土壤中微生物生物量C、N以及有机质含量显著高于常规栽培土壤。综上,有机栽培能够丰富活性细菌群落多样性,具有土壤优化效应。
Soil bacterial communities play an important role in vegetable cultivation. Based on the DNA and RNA levels, the diversity of soil bacterial community diversity and the relationship between soil physical and chemical properties and bacterial community diversity under different cultivation environments were studied by PCR-DGGE. The results showed that there was a significant difference in soil bacterial diversity under different cultivation methods. The dominant population and number of soil microorganisms were affected by organic, conventional and seasonal effects. Compared with conventional cultivation, organic cultivation could significantly increase the diversity of soil bacterial community. Cluster analysis showed that The diversity of 16S rDNA bacterial community was related to the season, while the diversity of 16S rRNA bacterial community was related to the cultivation method. The sequencing of the differential bands showed that the majority of bacterial species shared high homology with the uncultivable bacterial species, and the remaining 9 species were pseudo- According to CCA analysis, pH was the main factor affecting the diversity of soil bacterial community. The contents of C, N and organic matter in organic culture soil were significantly higher than those in conventional soil. In summary, organic cultivation can enrich the diversity of active bacterial community, with soil optimization effect.