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本文旨在研究染料木素(genistein)对体外培养的基底前脑胆碱能神经元发育的影响。取孕18d胎鼠基底前脑神经元,体外有血清培养7d后,随机分为3组:无血清培养液组(control组),genistein+无血清培养液组(G组)、E2+无血清培养液组(E2组)。48h后用MTT法测定细胞活力和代谢状态;用AChE组化染色以及ChAT和MAP2免疫荧光双重染色,镜下计数AChE阳性和ChAT阳性神经元数,并对两种神经元的细胞面积、第一级突起数及最长突起长度进行检测。结果显示:MTT法所测的OD值、AChE阳性和ChAT阳性神经元数、细胞面积、第一级突起数及最长突起长度在G组与control组之间无明显差异,然而E2组中的上述数值均明显增加。本研究的结果提示:genistein对体外培养的基底前脑胆碱能神经元无类似雌激素样的神经保护和营养作用。
This article aims to investigate the effects of genistein on the development of basal forebrain cholinergic neurons in vitro. The basal forebrain neurons of fetus and fetus were taken for 18 days. After being cultured in vitro for 7 days, they were randomly divided into 3 groups: control group, genistein + serum-free medium group (G), E2 + serum-free medium Group (E2 group). After 48h, cell viability and metabolic status were determined by MTT assay. AChE staining and double immunofluorescence staining of ChAT and MAP2 were used to count the number of AChE-positive and ChAT-positive neurons, and the cell area of the two neurons Level of the number of protrusions and the longest protrusion length of the test. The results showed that OD value, AChE positive and ChAT positive neurons, cell area, number of first-order protrusions and length of the longest protrusion measured by MTT method were not significantly different between G group and control group. However, in E2 group The above values were significantly increased. The results of this study suggest that genistein has no estrogen-like neuroprotective and nutritional effects on cultured basal forebrain cholinergic neurons in vitro.