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为解决丁型肝炎诊断试剂短缺的问题，本研究采用实验感染ＨＤＶ的土拨鼠肝脏和丁型肝炎病人血清，提取纯化丁型肝炎病毒抗原和抗体，制备了检测ＨＤＡｇ、抗－ＨＤ、ＩｇＭ抗－ＨＤ的系列酶免诊断试剂。该试剂与进口同类试剂相比较，符合率达１００％。其特异性好，灵敏度较高。对不同类型乙型肝炎病人血清检测，ＨＤＡｇ、抗－ＨＤ及ＩｇＭ抗－ＨＤ阳性率和总阳性率分别为２．９９％、３．３６％、３．７３％和７．２８％。该试剂的研制为丁型肝炎病毒感染的临床诊断及流行病学研究提供了可靠的实验手段。
In order to solve the problem of shortage of diagnostic reagent for hepatitis D, we tested the serum and anti-HDV, anti-HD and IgM anti-hepatitis B virus antibodies against hepatitis D virus (HDV) -HD series of enzyme-free diagnostic reagents. The reagent compared with imported similar reagents, in line with the rate of 100%. Its specificity is good and its sensitivity is high. The positive rate of HDAg, anti-HD and IgM anti-HD and the total positive rate were 2.99%, 3.36%, 3.73% and 7.28% for the serums of different types of hepatitis B patients. The development of this reagent provides a reliable experimental method for the clinical diagnosis and epidemiological study of hepatitis D virus infection.