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三点校正法是由Morton和Stubb于1946年为解决维生素A中不相关物质对紫外法测定的干扰而提出的,至今这种校正法仍被采用着。60年代Bastow应用三点校正法成功地测定了口服避孕片中有背景吸收的炔雌醇甲醚。近年来紫外分光光度法中涌现了多种消除干扰吸收的方法,其中被普遍接受的有三波长法。基于三点校正法与三波长法被平行使用的现状,本文首次从理论基础和计算公式上探讨了两方法的联系,以求用新观点对老方法再认识及使计算药学的方法更加系统规范。
The three-point calibration method was proposed by Morton and Stubb in 1946 to address the interference of UV-related determination of unrelated substances in vitamin A. To date, this method of calibration has been adopted. In the 60s, Bastow successfully measured ethinyl estradiol methyl ether in oral contraceptive tablets using the three-point calibration method. In recent years, UV spectrophotometry emerged a variety of ways to eliminate interference absorption, which is generally accepted by the three-wavelength method. Based on the current situation that the three-point calibration method and the three-wavelength method are used in parallel, this article discusses the connection between the two methods for the first time from the theoretical basis and the formula, in order to re-understand the old method with the new viewpoint and make the method of calculating the medicine more systematic and standard .