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寄主识别与附着胞分化是虫生真菌启动侵染过程的首要步骤。本文利用先前获得的金龟子绿僵菌基因缺失突变株与其野生型一起进行附着胞分化研究。接种后不同时间下的观察表明,绿僵菌突变株或野生型的附着胞既可以在萌发不久的芽管顶端形成,也可以在伸长菌丝分支的顶端形成。与野生型不同的是,突变株附着胞的分化频率显著下降,附着胞周围也缺乏粘液层的产生。研究表明,绿僵菌的类枯草杆菌类体壁降解酶对于附着胞分化不产生影响,对体壁降解也非完全必需的。与突变株附着胞分化频率显著降低相对应,其胞内环腺苷酸cAMP水平显著下降,而添加外源cAMP能够显著增加其附着胞分化频率,说明绿僵菌cAMP信号途径对于调控附着胞分化起着重要的作用。
Host identification and appressorial differentiation are the first steps in the initiation of infection by entomogenous fungi. In this paper, we used the previously obtained Metarhizium anisopliae gene deletion mutant with its wild-type appressorium differentiation study. Observation at different times after inoculation showed that the Metarhizium mutans or wild-type appressorium can be formed either on the apical germinating germ tube tip or on the apical side of the elongated hypha branch. In contrast to the wild type, the frequency of differentiation of the adherent cells in the mutant strain was significantly reduced, and the mucus layer was also lacking around the attachment cells. Studies have shown that Metarhizium anisopliae-like bacterium wall-degrading enzymes have no effect on appressorial differentiation and are not completely necessary for body wall degradation. Correspondingly, the level of cAMP in the intracellular cyclic adenosine monophosphate decreased markedly, and the addition of exogenous cAMP significantly increased the frequency of appressorial differentiation, indicating that the cAMP signaling pathway of Metarhizium anisopliae could play an important role in the regulation of appressorial differentiation It plays an important role.