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目的 为了模拟在体卵巢释放激素的生理功能 ,将成年大鼠的双卵巢切除后行自体腹腔移植 ,观察其移植效果 ,为临床卵巢移植提供实验依据 ,探索治疗围绝经期综合征的新途径。方法 将性成熟的 SD雌性大鼠的双侧卵巢切除 ,两个卵巢立即行自体腹腔移植 ,通过观察阴道脱落细胞变化、子宫角形态及测定血清雌激素水平 ,了解移植后大鼠的内分泌情况 ,判断移植成功与否以及内分泌功能如何。结果 移植组阴道脱落细胞 7/8显示有雌激素分泌 ,子宫角形态及血清的雌激素水平明显优于去势组 (P <0 .0 5 ) ,与正常组差异无显著性 (P>0 .0 5 )。结论 新鲜卵巢自体移植后 ,能分泌雌激素 ,并作用于靶器官维持其功能 ,为临床卵巢移植提供了实验依据。卵巢移植有可能成为临床治疗卵巢内分泌功能不足的新方法。
Objective To simulate the physiological function of hormone release in the ovary, adult ovariectomized rats were transplanted into the abdominal cavity to observe the transplanted effect and provide experimental basis for clinical ovarian transplantation to explore a new way to treat perimenopausal syndrome. Methods The bilateral ovariectomized female SD rats were ovariectomized and the two ovaries were transplanted into the abdominal cavity immediately. The changes of vaginal cells, the morphology of uterine horn and the level of serum estrogen were observed to understand the endocrine status of the rats after transplantation. Determine the success of transplantation and endocrine function. Results 7/8 vaginal exfoliated cells in the transplantation group showed estrogen secretion, hormones in the uterus and serum estrogen levels were significantly better than those in the castrated group (P <0.05), but no significant difference was found between the normal group and the ovariectomized group .0 5). Conclusion After ovarian autotransplantation, it can secrete estrogen and act on the target organs to maintain its function, providing experimental evidence for clinical ovarian transplantation. Ovarian transplantation may become a new method of clinical treatment of ovarian endocrine deficiencies.