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猴肝细胞膜蛋白经 SDS- PAGE和蛋白转移电泳后用免疫印迹法测定 ,硝酸纤维素膜分别与抗牛肾上腺皮质 LDL受体的抗体、LDL、Lp( a)和 PLG温育后见有 3条不同的蛋白条带 ,分子量分别为 370 kd、2 90 kd和 80 kd.用酶标法测定猴肝细胞膜蛋白 ,发现经 Lp( a) + PLG温育后用 Lp( a)抗体反应的结果与 Lp( a)温育后用 Lp( a)抗体反应的结果比较无显著差异 ,而经 Lp( a) + PLG温育后用 PLG抗体反应的结果比单独用 PLG温育后用 PLG抗体反应的作用强 ;同样经 Lp( a) +LDL温育后用 Lp( a)抗体反应的结果与经 Lp( a)温育后用 Lp( a)抗体反应的结果比较无显著差异 ,而经 Lp( a) + LDL温育后用 LDL抗体反应比单独经 LDL温育后用 LDL抗体的反应强 ,排除Lp( a)与 PLG和 LDL的交叉反应 ,实验认为猴肝细胞膜上可能同时存在 LDL受体、PLG受体和Lp( a)受体
The membrane proteins of monkey hepatocytes were determined by SDS-PAGE and protein transfer electrophoresis and Western blotting respectively. After nitrocellulose membrane was incubated with anti-bovine adrenocortical LDL receptor antibody, LDL, Lp (a) and PLG respectively, there were 3 The bands of different proteins were 370 kd, 290 kd and 80 kd, respectively. The membrane protein of monkey hepatocytes was determined by enzyme-linked immunosorbent assay. The result of Lp (a) + PLG incubated with Lp (a) There was no significant difference in Lp (a) antibody response after incubation with Lp (a), while the result of the reaction with PLG antibody after incubation with Lp (a) + PLG was greater than that of PLG antibody incubation with PLG alone The results of Lp (a) antibody reaction after incubation with Lp (a) + LDL were not significantly different from those of Lp (a) antibody incubated with Lp (a) a) + LDL incubated with LDL antibody response than LDL alone incubated with LDL antibody strong reaction to exclude Lp (a) and PLG and LDL cross-reaction, the experiment that the monkey liver cell membrane may exist at the same time LDL receptor , PLG receptor and Lp (a) receptor