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LY-04和WSQIF是由华中师范大学农药所合成的两种新型有机磷农药,LY-04属于α-氧代膦酸衍生物,WSQIF属于芳氧基乙酰氧基烃基膦酸脂。选用斜生栅藻(ScenedesmusobliquusKütz.)作为实验生物,通过显微计数测定藻细胞的密度以计算LY-04和WSQIF对斜生栅藻24h、48h、72h、96h的半效应抑制浓度(EC50),研究其对斜生栅藻的毒性效应。结果表明:以丙酮为溶剂时,每隔24h取样,测得LY-04对斜生栅藻的48h、72h、96h的EC50值分别为2942.5mg/L、239.7mg/L和65.0mg/L,而WSQIF的24h、48h、72h、96h的EC50值分别为15.9mg/L、53.0mg/L、98.0mg/L和28.8mg/L。LY-04和WSQIF分别作为杀虫剂和除草剂而研制,经活体研究表明,LY-04具有较强的杀虫活性,WSQIF则具有很强的除草活性,所得EC50值基本符合设计结果。根据毒性分级标准,LY-04和WSQIF的EC50值远大于3mg/L,其对非靶生物中的水生藻类都属于低毒,可推广应用。
LY-04 and WSQIF are two new types of organophosphorus pesticides synthesized by Pesticides of Central China Normal University. LY-04 belongs to α-oxophosphonic acid derivatives and WSQIF belongs to aryloxyacetoxy hydrocarbyl phosphonates. Scenedesmus obliquus Kütz was selected as the experimental organism. The density of algae cells was determined by microscopic counting to calculate the half-effect inhibitory concentration (EC50) of LY-04 and WSQIF on S. obliquus 24h, 48h, 72h and 96h, To study its toxic effects on Scenedesmus obliquus. The results showed that the EC50 of 48 hours, 72 hours and 96 hours of LY-04 was 2942.5 mg / L, 239.7 mg / L and 65.0 mg / L, respectively, when acetone was used as solvent. The EC50 values of WSQIF at 24h, 48h, 72h and 96h were 15.9mg / L, 53.0mg / L, 98.0mg / L and 28.8mg / L, respectively. LY-04 and WSQIF were developed respectively as insecticides and herbicides. The results of in vivo studies showed that LY-04 had strong insecticidal activity and WSQIF had strong herbicidal activity. The obtained EC50 value basically conformed to the design results. According to the toxicity classification standard, the EC50 values of LY-04 and WSQIF are much larger than 3mg / L, which are low toxicity to non-target organisms in aquatic algae and can be popularized and applied.