论文部分内容阅读
目的研究瘦素受体基因GIN223Arg与2型糖尿病合并肥胖的关系。方法运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定无亲缘关系且具有完整资料的武汉地区汉人351例(其中2型糖尿病合并肥胖组131例、2型糖尿病非肥胖组148例、正常对照组72例)。结果①2型糖尿病合并肥胖组基因型AA、AG、GG(分别为0.398、0.480和0.122)与2型糖尿病非肥胖组的(0.196、0.223和0.581)和正常对照组分别为(0.208、0.264和0.528)比较有显著性差异(P<0.01)。②2型糖尿病合并肥胖组等位基因分布频率A、G分别为0.642及0.358,与非肥胖组及正常对照组比较有统计学意义(P<0.01)。③应用多重回归分析(NominalRegression)2型糖尿病与临床各变量的关系发现,2型糖尿病GIN223Arg变异(由A突变G)与BMI、收缩压、舒张压(分别为P=0.001,P=0.032,P=0.037)相关。结论瘦素受体基因GIN223Arg变异可能与2型糖尿病合并肥胖和高血压相关。
Objective To study the relationship between leptin receptor gene GIN223Arg and type 2 diabetes with obesity. Methods The unrelated and complete data were collected from 351 Han patients (including type 2 diabetes mellitus and obesity group, 131 type 2 diabetes mellitus and non-obesity type 2 diabetes mellitus) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) 148 cases, 72 cases of normal control group). Results ① The genotypes of AA, AG, GG (0.398,0.480 and 0.122 respectively) and the non-obese group with type 2 diabetes (0.196,0.223 and 0.581) and the normal control group in type 2 diabetes mellitus were (0.208,0.264 and 0.528 ) Compared with significant difference (P <0.01). ② The frequencies of allele A and G in obese patients with type 2 diabetes mellitus were 0.642 and 0.358 respectively, which were significantly different from those in non-obese patients and normal controls (P <0.01). (3) The relationship between type 2 diabetes mellitus (GIN223Arg) and BMI, systolic blood pressure and diastolic blood pressure (P = 0.001, P = 0.032, P = 0.037). Conclusion The mutation of leptin receptor GIN223Arg may be associated with type 2 diabetes with obesity and hypertension.