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用聚合酶链反应（ＰＣＲ）检测１０８例病程在２月以上的久治不愈的性病患者的病原体，结果淋球菌ＤＮＡ阳性４４例（４０．７％）、沙眼衣原体ＤＮＡ阳性３６例（３３．３％）、解脲支原体ＤＮＡ阳性３０例（２７．８％），部分病例同时作淋球菌培养。沙眼衣原体免疫荧光检测及解脲支原体培养检出阳性率均低于ＰＣＲ法。淋球菌和支原体检测二法阳性率有非常显著性查异（Ｐ＜０．００１），衣原体检测二法阳性率无显著性差异（Ｐ＞０．０５）。本次检测使７６．９％的患者明确了病因学诊断，进行了针对性治疗，治愈率达８６．６～９２．３％。ＰＣＲ检测在久治不愈的性病患者中有临床应用价值。
The pathogen of 108 patients with STDs whose disease duration was longer than 2 months were detected by polymerase chain reaction (PCR). The results showed that Neisseria gonorrhoeae DNA was positive in 44 cases (40.7%) and Chlamydia trachomatis DNA was positive in 36 cases (33.3%). , Ureaplasma urealyticum DNA positive 30 cases (27.8%), in some cases at the same time for Neisseria gonorrhoeae culture. Chlamydia trachomatis immune fluorescence detection and detection of Ureaplasma urealyticum positive rate was lower than the PCR method. Neisseria gonorrhoeae and Mycoplasma test two positive rate of a significant difference (P <0.001), Chlamydia test two positive rate was no significant difference (P> 0.05). The test made 76.9% of the patients clear etiological diagnosis, targeted treatment, the cure rate was 86.6 ~ 92.3%. PCR testing in patients with venereal disease have clinical value.