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本文旨在研究异丙酚对大鼠不完全性脑缺血后兴奋性毒性介质及ca~(2+)超载的影响,从分子水平探讨其脑保护作用和可能的机制。材料与方法 40只SD大鼠随机分成无缺血对照组(Ⅰ);脑缺血30分钟组(Ⅱ);异丙酚给药脑缺血30分钟组(Ⅲ);脑缺血30分钟再灌注60分钟组(Ⅳ);异丙酚给药脑缺血30分钟再灌注60分钟组(Ⅴ)。大鼠脑不完全缺血再灌注模型参照有关文献制作。两异丙酚给药组以微量输注泵先给予2.0mg·kg~(-1)·min~(-1)异丙酚和1.0mg·kg~(-1)·min~(-1)维库溴铵,5分钟后异丙酚减为1.0mg·kg~(-1)·min~(-1)维持;非异丙酚组给予2.5mg·kg~(-1)·h~(-1)芬太尼和同上剂量的维库溴铵。整个实验中,各实验大鼠均以小动物呼吸机给予机械通气并以白炽灯照,保持肛温37℃。
The aim of this study was to investigate the effect of propofol on excitotoxicity and ca2 + overload in rats after incomplete cerebral ischemia, and to explore its neuroprotective effect and its possible mechanism from the molecular level. MATERIALS AND METHODS: Forty Sprague Dawley rats were randomly divided into three groups: Ischemia control group (I); Cerebral ischemia for 30 minutes (Ⅱ); Propofol administration for 30 minutes (Ⅲ); Cerebral ischemia for 30 minutes The rats were infused for 60 minutes (IV). Propofol was administered for 30 minutes and reperfusion for 60 minutes (Ⅴ). Rat model of incomplete ischemia reperfusion with reference to the literature. Propofol 2.0 mg · kg -1 · min -1 and propofol 2.0 mg · kg -1 · min -1 were first given to the two propofol groups, Vecuronium bromide was maintained at 1.0 mg · kg -1 · min -1 after 5 minutes and 2.5 mg · kg -1 · h -1 at the non-propofol group -1) Fentanyl and ibuprofen. Throughout the experiment, all experimental rats were ventilated with a small animal ventilator and irradiated with an incandescent lamp to maintain the rectal temperature at 37 ° C.