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为了研究碱性臭氧预处理对稻草秸秆酶水解、表观结构及成分的影响,将稻草秸秆碱性臭氧预处理后进行酶水解,对处理前后的稻草秸秆进行了扫描电镜观察及成分分析,并对处理后溶液进行了紫外光谱分析。结果表明:碱性臭氧预处理能将稻草秸秆中的木质素氧化降解为小分子的有机酸,降低了稻草秸秆中木质素的含量,提高了纤维素的含量。扫描电镜观察显示经碱性臭氧预处理过的稻草秸秆,机械组织暴露,孔隙度大,酶解的有效比表面积大。在pH值5.0、每单位底物加酶量31.2mg/g、45℃条件下,碱性臭氧预处理稻草秸秆酶水解120h时还原糖达到了902mg/g,糖化率为92.57%。在相同酶解条件下,碱性预处理与未处理稻草秸秆的糖化率分别为74.90%与53.53%。碱性臭氧预处理稻草秸秆的糖化率明显高于碱性预处理与未处理稻草秸秆的糖化率。
In order to study the effect of alkaline ozone pretreatment on enzymatic hydrolysis, apparent structure and composition of straw stalks, straw stalks were pretreated with alkaline ozone for enzymatic hydrolysis. Scanning electron microscopy and compositional analysis were performed on straw stalks before and after treatment The treated solution was analyzed by UV spectroscopy. The results showed that alkaline ozone pretreatment could oxidize lignin in straw stalks to small molecule organic acids, reduce the content of lignin in straw and increase the content of cellulose. Scanning electron microscopy showed that straw stalks pretreated with alkaline ozone were exposed to mechanical tissue with large porosity and large specific surface area for enzymolysis. At pH 5.0, the amount of enzyme added per unit substrate was 31.2 mg / g. Under the condition of 45 ℃, the reducing sugar reached 902mg / g and the saccharification rate was 92.57% under alkaline ozone pretreatment for 120h. Under the same conditions, the saccharification rates of alkaline pretreatment and untreated straw were 74.90% and 53.53%, respectively. Alkaline ozone pretreatment straw straw saccharification rate was significantly higher than the alkaline pretreatment and untreated straw straw saccharification rate.