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目的探讨归元口服液中主要成分之一鹿茸的鉴别方法,为质量标准提供科学依据。方法定性鉴别采用TLC法,含量测定采用HPLC法。结果TLC法鉴别,对照药材、供试品有相同颜色的棕红色斑点,阴性样品无干扰。HPLC法鉴别,测定鹿茸中的成分尿苷和次黄嘌呤,用ODS2C18色谱柱,流动相[甲醇-四氢呋喃(2∶1)]-水(0.1∶99.9)检测波长254nm,尿苷回收率为99.67%,RSD=2.23%;次黄嘌呤回收率为98.18%,RSD=2.14%。结论TLC-HPLC法可以作为归元口服液中鹿茸的质量控制方法之一,处方中鹿茸的投药量不少于5.0%时,尿苷、次黄嘌呤在供试品中含量的最低浓度分别高于0.130g/L和0.050g/L。
Objective To investigate the identification method of pilose antler, one of the main components of Guiyuan oral liquid, and provide scientific basis for quality standards. The method was qualitatively identified by TLC and the content was determined by HPLC. RESULTS: The TLC method was used to identify the reference materials and the test samples had brown-red spots of the same color, and the negative samples had no interference. Determination of uridine and hypoxanthine in pilose antler by HPLC, using ODS2C18 column, mobile phase [methanol-tetrahydrofuran (2:1)]-water (0.1:99.9) detection wavelength 254nm, uridine recovery was 99.67 %, RSD = 2.23%; Hypoxanthine recovery was 98.18%, RSD = 2.14%. Conclusion The TLC-HPLC method can be used as one of the quality control methods of pilose antler in Guiyuan oral liquid. When the dosage of antler in the prescription is not less than 5.0%, the lowest concentrations of uridine and hypoxanthine in the test sample are respectively high. At 0.130 g/L and 0.050 g/L.