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本文应用原位杂交技术在大鼠睾丸恒冷箱切片上研究了促甲状腺激素释放激素受体 (TRH- R) RNA的表达和定位。由 DNA合成仪合成两个含 48个碱基的寡核苷酸探针 ,两个探针分别与小鼠垂体 TRH- R 1 0 0 5 - 1 0 5 2和 1 332 - 1 379区段的c DNA互补 ,生物素在 5 末端标记寡核苷酸探针。结果显示 TRH - R寡核苷酸探针与其互补的 m RNA杂交信号集中在大鼠睾丸的间质细胞中 ,生精细胞无杂交信号。杂交信号的强度依探针浓度而增加。该结果表明 TRH可能通过自分泌调节生殖功能和发育 ,TRH- R作用途径可能与在垂体的作用类似。
In this study, we used in situ hybridization to study the expression and localization of thyrotropin-releasing hormone receptor (TRH-R) RNA in rat testicular cryostat sections. Two 48-base oligonucleotide probes were synthesized by a DNA synthesizer. The two probes were respectively linked with the pituitary TRH- R 1 0 0 5 - 1 0 5 2 and 1 332 - 1 379 segments c DNA Complement, Biotin Labeled Oligonucleotide Probes at 5-Terminus. The results showed that the hybridization signal of TRH - R oligonucleotide probe and its complementary m RNA was concentrated in the rat testicular stromal cells, and the spermatogenic cells had no hybridization signal. The intensity of the hybridization signal increases with probe concentration. This result indicates that TRH may regulate reproductive function and development through autocrine, and the TRH-acting pathway may be similar to that in the pituitary gland.