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采用胎龄 18天大鼠海马神经元进行培养 ,第 5天以黄嘌呤 -黄嘌呤氧化酶 (X- XO)体系建立氧自由基损伤模型 ,并分别给与人参皂甙 (Gin)、超氧化物歧化酶 (SOD)、Gin+ SOD,观察氧自由基对神经元的损伤及 Gin、SOD的保护作用 ,结果表明 :氧自由基组细胞死亡率明显高于正常组 (P<0 .0 1) ,各抗自由基组细胞死亡率接近正常组 (P>0 .0 5 ) ,自由基组神经元细胞器严重受损 ,脂褐素增多 ,核变形偏位 ,胞浆与胞核比例减少 ,而各抗自由基组上述损伤减轻 ,提示 Gin和 SOD可增强神经元抗氧化能力 ,减轻氧自由基对神经元的损害
The hippocampal neurons of 18-day-old rats were cultured. On the fifth day, the model of oxygen free radical injury was established by xanthine-xanthine oxidase (X-XO) system. Gin and superoxide (SOD) and Gin + SOD. To observe the effects of oxygen free radicals on the neuron injury and the protective effect of Gin and SOD, the results showed that the cell death rate in oxygen free radical group was significantly higher than that in normal group (P <0.01) Each anti-free radical cell death rate close to the normal group (P> 0.05), free radical group of neuronal organelles severely damaged, increased lipofuscin, nuclear deformation bias, the ratio of cytoplasm and nucleus decreased, and each Anti-free radical group of the above lessening of injury, suggesting that Gin and SOD can enhance neuronal antioxidant capacity, reduce oxygen free radicals damage to neurons