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目的:对不同产地了哥王药材中总黄酮的含量测定并进行比较。方法:样品制备后加5%亚硝酸钠溶液1mL,放置6min后,加10%硝酸铝溶液1mL,放置6min后,加4%氢氧化钠溶液10mL,15min后,甲醇定容至刻度线,摇匀,在波长λ=510nm下测定吸光度,以芦丁做对照。结果:江西、广西、湖南3个产地的了哥王药材中的总黄酮平均含量分别为2.19%、2.52%、1.72%,广西产4批药材中含量相差较小。结论:可见不同产地,因不同的生长环境、温度、湿度等因素对了哥王中总黄酮的含量影响较大。
Objective: To determine the contents of total flavonoids in different origins of Costa Rica and compare them. Methods: Add 1 mL of 5% sodium nitrite solution after sample preparation, place for 6 min, add 1 mL of 10% aluminum nitrate solution, place for 6 min and add 10 mL of 4% sodium hydroxide solution. After 15 min, dilute the methanol to the mark and shake Uniform, measured at a wavelength λ = 510nm absorbance to rutin as a control. Results: The average content of total flavonoids in Gordoniae officinalis from three producing areas in Jiangxi, Guangxi and Hunan was 2.19%, 2.52% and 1.72%, respectively. The contents of four flavonoids in Guangxi were less different. Conclusion: It can be seen that the content of total flavonoids in Columbia King has a great influence on the content of total flavonoids in different producing areas due to different growth environment, temperature, humidity and other factors.