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目的:探讨过表达大鼠醛酮还原酶AKR7A1蛋白对巴豆醛致畸作用的影响。方法:使用Western blot法和醛酮还原酶(AKR)酶活性技术检测并鉴定外源性AKR7A1在V79-4细胞中的表达水平和催化活性,使用HGPRT基因突变实验检测在V79-4细胞中过表达AKR7A1对巴豆醛致畸作用的影响。结果:Western blot检测显示V79-4细胞中表达高水平的AKR7A1蛋白;AKR酶活性实验结果显示过表达的AKR7A1蛋白具有催化活性;HGPRT基因突变实验结果显示过表达AKR7A1的V79-4细胞对巴豆醛致畸作用的抵抗力明显高于对照细胞。结论:过表达大鼠AKR7A1能显著提高V79-4细胞对巴豆醛致畸作用的抵抗力。
Objective: To investigate the effect of overexpressing rat aldoketokinase AKR7A1 on the teratogenicity of crotonaldehyde. Methods: The expression level and catalytic activity of exogenous AKR7A1 in V79-4 cells were detected and identified by Western blot and enzyme-linked aldehyde-ketone reductase (AKR) enzyme activity assay. HGPRT gene mutation assay was used to detect the expression of exogenous AKR7A1 in V79-4 cells Effect of expressing AKR7A1 on teratogenicity of crotonaldehyde. Results: The results of Western blot showed that the AKR7A1 protein was expressed in V79-4 cells. The results of AKR enzyme activity showed that AKR7A1 protein was overexpressed. The HGPRT gene mutation assay showed that V79-4 cells overexpressing AKR7A1 could inhibit the activity of crotonaldehyde Teratogenic effects of resistance was significantly higher than the control cells. Conclusion: Overexpression of rat AKR7A1 can significantly enhance the resistance of V79-4 cells to teratogenicity of crotonaldehyde.