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正常肝信息核糖核酸是从肝聚核蛋白体抽提RNA,通过寡聚(dT)纤维素亲和层析制备。分离的正常肝mRNA在麦胚蛋白合成系统和爪蟾卵翻译系统检定,能够翻译白蛋白,由此证明是有生物学活性的。用正常肝mRNA在离体情况下对肝癌细胞进行逆转分化研究,发现:(1)小鼠正常肝mRNA能抑制小鼠腹水肝癌细胞核酸和蛋白质的合成;初步见到人的正常肝mRNA能轻度抑制人体肝癌细胞(BEL-7404)的生长。(2)相应的正常肝mRNA分别在小鼠腹水肝癌和人肝癌细胞诱导了白蛋白合成;在人体肝癌细胞内核蛋白体聚成聚核蛋白体。(3)人体肝癌细胞受刀豆凝集素(Con A)凝集的能力减弱,这种凝集特性的改变,可以维持至3次细胞传代。这些实验结果显示肝癌细胞并非固定不可改变的,在正常肝mRNA作用下能够向正常逆转分化;讨论了mRNA转化机理,认为可能是通过肝mRNA翻译的蛋白质或mRNA本身直接调节基因转录来实现的。
Normal hepatic RNA is extracted from hepatic ribosome and prepared by oligo(dT) cellulose affinity chromatography. The isolated normal liver mRNA was assayed in the wheat germ protein synthesis system and the Xenopus egg translation system and was capable of translating albumin, thereby demonstrating biological activity. Using normal liver mRNA to reverse the differentiation of hepatoma cells in vitro, we found that: (1) Normal liver mRNA of mice can inhibit the synthesis of nucleic acid and protein in mouse ascites liver cancer cells; it is initially seen that human normal liver mRNA can be light Inhibits the growth of human hepatoma cells (BEL-7404). (2) Corresponding normal liver mRNA induces albumin synthesis in mouse ascites liver cancer and human hepatoma cells, respectively; in human hepatoma cells, the core protein bodies are polynucleated. (3) The ability of human hepatoma cells to agglutinate by Con A aggregates, and this change in agglutination properties can be maintained up to 3 cell passages. These experimental results show that hepatoma cells are not fixed and can be reverted to normal under the action of normal hepatic mRNA. The mechanism of mRNA conversion was discussed, and it is thought that the protein or mRNA itself translated by liver mRNA may directly regulate gene transcription.