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目的:通过对DMD鼠模型肌纤维的病理组化研究,来观察变性肌组织胶原纤维中的表达。方法:首先选取高纯度种族的小白鼠,进行分组,设为试验组和对照组,试验组将dystrophin基因敲除,使其发生DMD,然后对鼠模型的腓肠肌内侧部进行肌肉活检,使用HE染色,利用偏振光显微镜区分I型胶原纤维(红色或黄色)和III型胶原纤维(绿色)的表现情况。结果:DMD鼠模型中I型胶原纤维在变性肌组织中有明显的增多。结论:DMD的肌肉病理显示,存在慢性免疫原性炎症,考虑对于疾病的发生发展起到一定作用。
OBJECTIVE: To observe the expression of collagen fibers in degenerative muscle tissue by studying the histopathology of muscle fiber in DMD mouse model. Methods: First, the mice of high purity race were selected and divided into the experimental group and the control group. The dystrophin gene was knocked out in the experimental group to produce DMD. Then, the muscular biopsy was performed in the medial part of the gastrocnemius muscle of the mouse model and HE staining , Polarization microscopy was used to differentiate between type I collagen fibers (red or yellow) and type III collagen fibers (green). Results: Type I collagen fibers in DMD mouse model significantly increased in degenerative muscle tissue. CONCLUSIONS: The muscle pathology of DMD shows chronic immunogenic inflammation and is thought to play a role in the development of the disease.