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采用大孔树脂及中压液相色谱对蓝莓果渣酸乙醇提取液中花色苷进行纯化。结果表明:AB-8大孔树脂具有较好的吸附解吸特性,适合蓝莓果渣花色苷的初级纯化。最佳吸附条件为上样溶液浓度0.8 mg/mL,最佳吸附pH2.0,上样速率1.0 mL/min;最佳解吸条件为乙醇体积分数60%,解吸pH1.0,洗脱速率1.0 mL/min。经大孔树脂纯化,其纯度为38.49%。然后经中压液相色谱纯化,花色苷纯度提高到94.42%。此法简便、高效,能制得高纯度的花色苷。
The macroporous resin and medium pressure liquid chromatography were used to purify the anthocyanins in the blueberry fruit residue ethanol extract. The results showed that: AB-8 macroporous resin has good adsorption and desorption characteristics, suitable for the primary purification of blueberry pomace anthocyanins. The optimal adsorption conditions were as follows: the concentration of sample solution was 0.8 mg / mL, the optimum pH was 2.0 and the sample loading rate was 1.0 mL / min. The optimal desorption conditions were ethanol volume fraction 60%, desorption pH 1.0 and elution rate 1.0 mL / min. Purified by macroporous resin, the purity was 38.49%. Then by medium pressure liquid chromatography purification, anthocyanin purity increased to 94.42%. This method is simple and efficient, can produce high purity anthocyanins.