论文部分内容阅读
目的：探讨人乳头瘤病毒（ Ｈ Ｐ Ｖ） Ｄ Ｎ Ａ 在原发性肺癌组织中的表达． 方法：利用西京医院病理科２７５ 例肺癌病理标本进行研究，以同期３２ 例支气管粘膜鳞状化生和３４ 例粘膜慢性炎症标本作为双非肿瘤对照组． 采用随机引物法制备地高辛标记 Ｈ Ｐ Ｖ６ Ｂ／１１，１６，１８，３１ 型 Ｄ Ｎ Ａ 探针，应用原位杂交检测肺癌组织中的 Ｈ Ｐ Ｖ Ｄ Ｎ Ａ 存在情况． 结果： Ｈ Ｐ Ｖ 原位杂交阳性信号位于组织细胞核内． 肺癌 Ｈ Ｐ Ｖ 感染总阳性率为３３．８％ （９３／２７５）而各细胞类型阳性率为：肺鳞癌 ４８．５％ ，腺癌１８０％ ，小细胞癌２０２％ ，大细胞肺癌３３．３％ ，鳞状上皮化生不典型增生２１．９％ ，粘膜慢性炎症５．９％ ． 以粘膜炎症组为对照，肺鳞癌阳性率最高，与对照组相比差异性显著． 肺鳞癌组织中 Ｈ Ｐ Ｖ 各型阳性率 Ｈ Ｐ Ｖ６ Ｂ／１１ 为 ２１． ６％ ， Ｈ Ｐ Ｖ１６ 为２０２％ ， Ｈ Ｐ Ｖ１８ 为２３．８％ ， Ｈ Ｐ Ｖ３１ 为１９．０％ ， 各型之间差异不显著． 结论：肺鳞癌、腺癌、小细胞癌、大细胞癌中均存在不同程度 Ｈ Ｐ Ｖ 感染，肺鳞癌与 Ｈ Ｐ Ｖ 感染关系密切．
4. Objective : To investigate the expression of human papilloma virus (NPV) D N A in primary lung cancer. METHODS: A total of 275 cases of pathological specimens of lung cancer from the Department of Pathology, Xijing Hospital were used in the study. 32 cases of squamous metaplasia of bronchial mucosa and 34 cases of mucosal chronic inflammation were used as non-tumor control group. Digoxigenin-labeled HDV B/11,16,18,31 D N A probes were prepared by random primer method. The presence of H pv D N A in lung cancer tissues was detected by in situ hybridization. Results: The positive signal of H P V in situ hybridization was located in the nucleus of the tissue. The overall positive rate of H p V infection in lung cancer was 33.8% (93/275) and the positive rate of each cell type was: 48.5% of lung squamous cell carcinoma, 18% of adenocarcinoma, and 20. 2% of small cell carcinoma. Cell lung cancer 33.3%, squamous metaplasia atypical hyperplasia 21.9%, mucosal chronic inflammation 5.9%. In the mucosal inflammation group as the control, the positive rate of lung squamous cell carcinoma was the highest, and the difference was significant compared with the control group. The positive rate of HPV types in lung squamous cell carcinoma tissues was H P V6 B/11 21. 6%, H P V16 was 202%, H P V18 was 23.8%, H P V31 was 19.0%, and there was no significant difference among various types. Conclusion: There are different levels of H P V infection in lung squamous cell carcinoma, adenocarcinoma, small cell carcinoma and large cell carcinoma. Lung squamous cell carcinoma is closely related to H P V infection.