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对 Cd Cl2 和 Cd Cl2 + α- h CRF(α螺旋促肾上腺皮质激素释放因子 )分别染毒的 SD大鼠 ,用 [3H-Td R]掺入法测定大鼠脾 T、B淋巴细胞增殖转化功能 ,垂体前叶细胞培养法测定中枢下丘脑和外周血浆中的 CRF含量。结果表明单独染镉组有丝分裂原诱导的鼠脾 T、B淋巴细胞增殖转化均受到明显抑制 ,具有剂量 -效应关系 (P<0 .0 5 ) ;动物下丘脑 CRF含量伴随着染镉剂量升高 (P<0 .0 5 ) ;外周血浆 CRF含量各组间差异无显著性 (P>0 .0 5 )。当使用拮抗剂 α- h CRF后 ,在含镉染毒各剂量组间鼠脾 T、B细胞功能抑制差异无显著性 (P>0 .0 5 ) ;下丘脑 CRF含量在各含镉联合染毒组间差异无显著性 (P>0 .0 5 ) ,但均较对照组高 (P<0 .0 5 ) ;外周血浆 CRF水平有升高趋势。结果提示单独染镉可引起下丘脑 CRF含量升高和免疫功能抑制 ,联合使用 α- h CRF后则能部分改善镉对大鼠脾细胞的免疫功能抑制。镉对大鼠脾淋巴细胞免疫毒作用过程中存在中枢 CRF介导的免疫调节机制
Splenic T, B lymphocyte proliferation and transformation of rat spleen were measured by [3H-Td R] incorporation method in SD rats exposed to Cd Cl2 and Cd Cl2 + α-h CRF (α-helical corticotropin releasing factor) Function, anterior pituitary cell culture assay for determination of CRF content in the central hypothalamus and peripheral plasma. The results showed that mitogen-induced T and B lymphocyte proliferation and transformation in the splenocytes of cadmium-alone group were significantly inhibited, with a dose-effect relationship (P <0.05); the content of CRF in the hypothalamus of animals was increased with the increase of cadmium dose (P <0.05). There was no significant difference in peripheral blood plasma CRF levels among all groups (P> 0.05). When antagonist α-h CRF was used, there was no significant difference in the inhibitory effect of T and B cell proliferation between groups with or without cadmium (P> 0.05) There was no significant difference between the two groups (P> 0.05), but both were higher than those in the control group (P <0.05). The level of CRF in the peripheral blood was increased. The results suggest that cadmium alone can induce hypothalamic CRF content and immune function inhibition, combined with α-h CRF can partially improve the immune function of rat spleen cells inhibited by cadmium. Central CRF Mediated Immunomodulatory Mechanism in the Immunotoxicity of Cadmium on Rat Spleen Lymphocytes