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目的:建立寒湿痹片的质量标准。方法:采用显微鉴别法鉴别处方中的细辛;采用薄层色谱法对处方麻黄,甘草,桂枝,黄芪进行定性鉴别;采用高效液相色谱法对马兜铃酸A进行限量检查;采用高效液相色谱法测定芍药苷的含量。结果:显微特征明显;薄层色谱中可检出麻黄,甘草,桂枝,黄芪,阴性均无干扰;限量检查中未检查出马兜铃酸A,符合规定;芍药苷进样量在(0.029185~2.3348μg)范围内,线性关系(r=0.9999)良好。本方法平均回收率为99.4%,RSD为1.5,(n=6)。结论:该方法准确,重复性好,可用于寒湿痹片的质量控制。
Objective: To establish the quality standard of alpine film. Methods: The prescription of Asarum was identified by microscopic identification method. The prescriptions of ephedra, licorice root, cassia twig and astragalus membranaceus were qualitatively identified by TLC. The content of aristolochic acid A was determined by HPLC. Determination of Paeoniflorin by High Performance Liquid Chromatography. Results: The microscopic features were obvious. There was no interference of ephedra, licorice root, cassia twig, astragalus membranaceus, and feminine in thin layer chromatogram. No detection of aristolochic acid A was found in the limited inspection. The dosage of paeoniflorin was (0.029185 ~ 2.3348μg), the linear relationship (r = 0.9999) is good. The average recovery of this method was 99.4% with a RSD of 1.5 (n = 6). Conclusion: The method is accurate and reproducible, and can be used for quality control of alpine film.