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目的 分析原发性非小细胞肺癌（ＮＳＣＬＣ） 中ｐ５３ 基因第５ ～８ 外显子点突变发生及其意义。方法 运用聚合酶链反应－ 单链构象多态性分析方法（ＰＣＲＳＳＣＰ 分析法） 进行检测。结果 发现８ 例肺良性疾病均无点突变发生，４０ 例ＮＳＣＬＣ 中１９例（４７ ．５ ％ ） 有点突变发生，点突变发生与性别，年龄，组织学类型和组织学分级无明显相关性（ Ｐ＞ ０ ．０５） ，但与临床分期和淋巴结累及状态明显相关（ Ｐ＜ ０ ．０１ ，Ｐ＜ ０ ．０５） 。结论 结果表明ｐ５３ 基因点突变在ＮＳＣＬＣ 的发生和进展中起着相当重要的作用。
Objective To analyze the occurrence and its significance of point mutations in exon 5 to exon 8 of p53 gene in primary non-small cell lung cancer (NSCLC). Methods Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP analysis) was used to detect. There was no point mutation in 8 cases of benign lung diseases. There were some mutations in 19 cases (47.5%) of 40 cases of NSCLC. There was no significant correlation between point mutation and gender, age, histological type and histological grade > 0 .05), but significantly correlated with clinical stage and lymph node involvement (P <0.01, P <0.05). Conclusion The results show that p53 gene point mutation plays a very important role in the occurrence and progression of NSCLC.