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本文应用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶组织化学方法和FOS免疫组化法相结合的双重反应技术，研究了大鼠腰骶髓内的NOS阳性结构的分布及其与膀胱伤害性刺激诱发的FOS表达神经元的关系。结果发现NOS阳性纤维和终末密集分布于后角Ⅰ层和Ⅱ层，其它部位稀少；NOS阳性神经元胞体与膀胱伤害性刺激诱发的c-fos原癌基因蛋白表达的阳性神经元在腰骶髓（L6～S1）分布区域校相似，主要分布于与伤害性信号传递过程密切相关的后角Ⅱ层、中央管周围灰质（包括骶髓后连合核）和中间带外侧核．在此三部位分别有18．7％、11．6％和22．6％和NOS阳性神经元同时呈FOS阳性；有5．56％、2．67％和3％FOS阳性神经元同时是NOS阳性。结合文献和本文结果讨论了NO可能参与脊髓伤害性信号传递和调控的作用．
In this paper, the use of reduced nicotinamide adenine dinucleotide dehydrogenase histochemical method and FOS immunohistochemistry combined with dual reaction technology to study the rat lumbosacral NOS-positive distribution of the structure and the bladder injury Stimulation-induced FOS expression in neurons. The results showed that NOS-positive fibers and terminals were intensively distributed in the posterior horn of Ⅰ and Ⅱ layers, other parts of the sparse; NOS positive neurons and bladder nociceptive stimulation of c-fos protooncogene protein expression positive neurons in the lumbosacral The distribution regions of the marrow (L6 ~ S1) are similar, mainly distributed in the posterior horn Ⅱ layer which is closely related to the nociceptive signaling process, the peripheral gray matter of the central canal (including the sacral spinal dorsal commissure) and the medial lateral ventricle. In these three sites, 18.7%, 11.6% and 22.6% of the NOS positive neurons were FOS positive at the same time; while 5.56%, 2.67% and 3% FOS positive neurons were both NOS Positive. Combined with the literature and results of this article discusses the role of NO may be involved in spinal cord nociceptive signaling and regulation.