论文部分内容阅读
测定了筛选木霉菌株NF9和TC3对喀麦隆芋艿根腐病病原真菌的体外拮抗作用。在对峙培养中,NF9和TC3对群结腐霉(Pythiummyriotylum)(Pm),立枯丝核菌(Rhizoctoniasolani)(Rs)和茄镰孢(Fusariumsolani)(Fs)的拮抗系数分别为1—2,1,1.在赛珞玢分层培养中,NF9对Pm,Rs,Fs的菌丝生长抑制率分别为100%,93.4%及55.3%;而TC3对上述真菌的抑制率分别为100%,92.1%及46.4%,光学显微镜下观察到NF9对Rs菌丝的缠绕和穿透;在不同温度(10—35℃)、不同酸度(pH3—9)条件下,在PDA培养基上,NF9的菌丝生长均明显超过群结腐霉(Pm).当PDA培养基中瑞毒霉(Ridomil)的含量为23mg/L及46mg/L时,群结腐霉的生长完全受到抑制,而木霉菌株NF9和TC3的生长甚至超过对照(不含瑞毒霉).实验结果表明了上述木霉菌株在病害防治中的应用潜力。
In vitro antagonism of Trichoderma strains NF9 and TC3 against root rot pathogens of taro root rot in Cameroon was determined. In confrontation culture, the antagonistic coefficients of NF9 and TC3 against Pythium myriotylum (Pm), Rhizoctonia solani (Rs) and Fusarium solani (Fs) were 1-2, 1,1. The inhibitory rates of NF9 on the mycelial growth of Pm, Rs and Fs were 100%, 93.4% and 55.3%, respectively. The inhibitory rates of TC3 to these fungi were 100%, 100% 92.1% and 46.4% respectively. The entanglement and penetration of NF9 to Rs mycelia were observed under light microscope. Under the conditions of different temperature (10-35 ℃) and different acidity (pH 3-9) On the other hand, mycelial growth of NF9 was significantly higher than that of Pythium pyogenes (Pm). When Ridomil in PDA medium was 23 mg / L and 46 mg / L, the growth of P. harzianum was completely inhibited, whereas the Trichoderma strains NF9 and TC3 grew even more than the control mold). The experimental results show that the Trichoderma strains in the application of disease control potential.