论文部分内容阅读
目的:探究胶体金法检测在梅毒抗体检测中的应用以及弱阳性影响因素。方法:随机选取贵阳中医学院第二附属医院2015年5月至2016年4月收治的均经甲苯胺红不加热血清学试验(TRUST)检测确诊的89例梅毒患者,首先,准备TP–EHSA抗体诊断试剂盒(梅毒酶联免疫吸咐试验)、梅毒螺旋体明胶凝集试验诊断试剂盒以及胶体金法检测试剂盒;其次,组织受检者采集血样并处理;最后,根据试剂盒说明书进行梅毒检测,分析p H值、溶血、脂血对检测结果弱阳性的影响。结果:在本次检测过程中,胶体金法检测阳性56例(62.9%),弱阳性24例(27.0%),胶体金法诊断正确率为89.9%,阴性9例,漏诊率为10.1%。低浓度梅毒抗体标本溶血、脂血后容易出现弱阳性;p H为6.0~8.0时,胶体金法检测结果较准确。
Objective: To explore the application of colloidal gold assay in the detection of syphilis antibodies and the influencing factors of weak positive. Methods: A total of 89 syphilis patients diagnosed by TRUST were randomly selected from the Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine from May 2015 to April 2016. At first, TP-EHSA antibody Diagnosis kit (syphilis ELISA), Treponema pallidum agglutination test diagnostic kit and colloidal gold assay kit; secondly, tissue samples were collected and processed; finally, according to kit instructions for syphilis testing, Analysis p H value, hemolysis, lipids on the weak positive test results. Results: Colloidal gold detection was positive in 56 cases (62.9%) and weakly positive in 24 cases (27.0%) in this test. The accuracy rate of colloidal gold method was 89.9% and negative in 9 cases, with a missed diagnosis rate of 10.1%. Low concentration of syphilis antibody hemolytic, lipid-prone prone to weak positive; p H 6.0 ~ 8.0, colloidal gold test results more accurate.