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目的研究常用化疗药物对人肝癌细胞ＳＭＭＣ７７２１端粒酶活性的影响．方法利用端粒重复扩增实验（ＴＲＡＰ）结合非变性聚丙烯酰胺凝胶电泳银染法，测定ＳＭＭＣ７７２１细胞经过几种化疗药物（顺铂、阿霉素、丝裂霉素、５ＦＵ）不同浓度和时间作用后端粒酶活性的变化．结果当各种药物大剂量处理细胞４ｈ后再祛除药物培养２０ｈ后，顺铂对酶的活性完全抑制，丝裂霉素有部分抑制，阿霉素和５ＦＵ无抑制作用；而未经２０ｈ再培养则无作用；小剂量药物处理细胞５ｄ，其结果同大剂量相似．结论顺铂和丝裂霉素对人肝癌ＳＭＭＣ７７２１细胞端粒酶活性有抑制作用，可能与药物的作用机制有关．
Objective To study the effect of chemotherapeutic drugs on the telomerase activity of human hepatocellular carcinoma cell line SMMC7721. Methods The telomere repeat amplification assay (TRAP) combined with non-denaturing polyacrylamide gel electrophoresis silver staining was used to determine the SMMC 7721 cells after several chemotherapy drugs (cisplatin, doxorubicin, mitomycin, 5 FU ) Changes in telomerase activity after different concentrations and time. Results When various drugs were treated with large doses of cells for 4 hours and then cured for 20 hours, the activity of cisplatin was completely inhibited. Mitomycin was partially inhibited and doxorubicin and 5FU were not inhibited. No effect of re-culture; small doses of drug treatment of cells 5d, the results with a large dose similar. Conclusion cisplatin and mitomycin on human hepatocellular carcinoma SMMC 7721 cell telomerase activity inhibition may be related to the mechanism of drug action.