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用出生第３ｄＳｐｒａｇｕｅ－Ｄａｗｌｅｙ大鼠腹腔注射３％苯丙氨酸（Ｐｈｅ）液７ｄ，诱导高苯丙氨酸血症，并用聚酰胺薄膜层析荧光扫描法测定脑内氨基酸递质含量。结果显示，注射组大鼠血清中的Ｐｈｅ浓度为对照组的１８．２倍，大脑皮质中Ｐｈｅ浓度为对照组的２９．５倍；其小脑、大脑皮层及脑干中ｒ－氨基丁酸（ＧＡＢＡ）含量分别较对照组降低为２８．６％、３５．１％和３６．７％（Ｐ＜０．０５）；甘氨酸含量则升高约８０％～１２０％。大脑皮层突触体中，谷氨酸（Ｃｌｕ）、天门冬氨酸（Ａｓｐ）、牛磺酸（Ｔａｕ）及甘氨酸（Ｇｌｖ）、ＧＡＢＡ等含量均有不同程度降低（Ｐ＜０．０５）。可见，高苯丙氨酸血症大鼠对脑内递质氨基酸含量的影响，在不同脑区其影响程度也不同。
Hyperglycerolaemia was induced by intraperitoneal injection of 3% phenylalanine (Phe) solution for 3 days on the 3rd day of Sprague-Dawley rats, and the content of amino acid neurotransmitters in the brain was measured by polyamide thin-film chromatography. The results showed that the concentration of Phe in the serum of the injected group was 18.2 times that of the control group and the Phe concentration in the cerebral cortex was 29.5 times that of the control group. The contents of r-aminobutyric acid GABA) were reduced by 28.6%, 35.1% and 36.7% (P <0.05) respectively compared with the control group, while the content of glycine increased by about 80% -120%. Cerebral cortex synaptosomes, glutamic acid (Clu), aspartic acid (Asp), taurine (Tau) and glycine (Glv), GABA content were reduced to varying degrees (P <0.05). Can be seen, high phenylalaninemia rat brain neurotransmitter amino acid content in different brain regions its impact is also different.