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为建立保健食品的快速安全性评价方法,用CHO和V79两个细胞系对三七进行了MTT法、中性红吸收法、集落形成法进行细胞毒性试验,用BalBc细胞、CHL细胞、SL-7细胞3个细胞系对川芎用上述3种方法进行了细胞毒性试验。结果发现用CHO和CHL细胞系,中性红吸收法做出的细胞毒性结果比较稳定可靠。
To establish a rapid safety evaluation method of health food, MTT assay, neutral red absorption assay and colony formation assay were used to test the cytotoxicity of Panax notoginseng to Panax notoginseng by CHO and V79 cell lines. BalBc cells, CHL cells, SL- 7 cells 3 cell lines Chuanxiong cytotoxicity test using the above three methods. The results showed that with the CHO and CHL cell lines, neutral red absorption cytotoxicity results made more stable and reliable.