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目的：通过检测脑肿瘤杂合性丢失（ＬＯＨ）的集中区域协助有关肿瘤抑制基因（ＴＳＧ）的查找。方法：选取９个微卫星多态标记，以 ＰＣＲ扩增－变性测序凝胶电泳检测 ６７对瘤标本和相应正常组织ＤＮＡ。结果：胶质瘤在１３ｑ、１９ｑ和 ２２ｑ等位点存在较高频率（＞２０％）的ＬＯＨ，脑膜瘤在２２ｑ和１ｐ出现高频率（＞３０％）的ＬＯＨ，神经鞘瘤仅在２２ｑ位点出现４０％的ＬＯＨ。结论：脑肿瘤高频率的染色体位点特异性ＬＯＨ与ＴＳＧｓ的失活密切相关。
OBJECTIVE: To assist in the search of tumor suppressor genes (TSGs) by detecting the concentration of loss of heterozygosity (LOH) in brain tumors. Methods: Nine microsatellite polymorphism markers were selected to detect 67 pairs of tumor specimens and corresponding normal tissues by PCR amplification and denaturing sequencing gel electrophoresis. RESULTS: Gliomas showed high frequency (> 20%) of LOH at 13q, 19q and 22q sites, high frequency (> 30%) LOH of meningiomas at 22q and 1p, schwannoma only at 22q Point appears 40% of LOH. Conclusion: The high frequency chromosomal site-specific LOH in brain tumors is closely related to the inactivation of TSGs.