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本文采用原位杂交组织化学技术，结合计算机图象处理系统，对电针后大鼠脑内前脑啡肽原（ＰＰＥ）ｍＲＮＡ的表达进行半定量的观察。ＰＰＥｍＲＮＡ探针以半抗原的地高辛标记。电针１０小时之后，ＰＰＥｍＲＮＡ阳性信号的强度和神经元内合ＰＰＥｍＲＮＡ的面积在许多与痛和镇痛有关的核团明显增加，如尾核，伏核，视前区，中脑的脚间核，导水管周围灰质，黑质，红核等。表明电针促进了ＰＰＥｍＲＮＡ在大鼠脑内的表达。这可能是针刺具有累积和长期效应的机制之一。
In this study, in situ hybridization and histochemical techniques were used to detect the expression of proendorphin (PPE) mRNA in rat brain after electroacupuncture with semi-quantitative observation. The PPE mRNA probe is labeled with digoxigenin for the hapten. After 10 hours of electroacupuncture, the intensity of PPE mRNA positive signal and the area of PPE mRNA in neurons increased significantly in many pain and analgesic related nuclei, such as caudate nucleus, nucleus accumbens, preoptic area and midbrain. , Around the aqueduct gray matter, substantia nigra, red nucleus and so on. It shows that electroacupuncture promotes the expression of PPEmRNA in rat brain. This may be one of the mechanisms by which acupuncture has cumulative and long-term effects.