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应用免疫组织化学和图像分析方法,对34例胃印戒细胞癌及其癌旁球样异形增生细胞(GDC)的PCNA表达和DNA含量进行检测,并以15例正常胃粘膜组织作为对照组。结果:1.印戒细胞癌,Ⅰ、Ⅱ、Ⅲ级球样异形增生细胞PCNA阳性细胞指数依次为(74.6±8.2)％,(12.5±8.7)％,(43.3±14.9)％和(68.1±9.1)％。印戒细胞癌,Ⅱ、Ⅲ级GDC分别与对照组(6.5±3.3)％比较有显著性差异(P<0.01)。2.在DNA直方图上,正常胃粘膜呈位于2C区的单峰型。SCC的众数峰右移,分布分散,各例都有>5C的非整倍体细胞。GDC图像介于二者之间,并且Ⅲ级GDC有>5C非整倍体细胞出现。我们认为GDC是异常增生的细胞,是胃印戒细胞癌的癌前病变。
Immunohistochemical and image analysis methods were used to detect the expression of PCNA and DNA content in 34 cases of gastric signet ring cell carcinoma and adjacent nodular allograft cells (GDC), and 15 normal gastric mucosa tissues were used as the control group. Results: 1. Signet ring cell carcinoma, PCNA-positive cell index of type I, II, III balloon-like dysplasia cells were (74.6±8.2)%, (12.5±8.7)%, (43.3±14.9)%, and (68.1±) 9.1)%. Signet-ring cell carcinoma, GDCs of grades II and III were significantly different from the control group (6.5±3.3)%, respectively (P<0.01). 2. On the DNA histogram, normal gastric mucosa presented a unimodal pattern in the 2C zone. The peaks of the SCC peaks shift to the right and are distributed. Each case has >5C aneuploid cells. The GDC image is somewhere in between, and Grade III GDCs have >5C aneuploid cells. We believe that GDC is an abnormally proliferating cell and is a precancerous lesion of gastric signet ring cell carcinoma.