论文部分内容阅读
目的 研究胸腔积液端粒酶活性在良恶性胸腔积液中的鉴别诊断价值 ,并与癌胚抗原(CEA)进行对比。方法 用聚合酶链反应 酶联免疫吸附分析法 (PCR ELISA)检测胸腔积液端粒酶活性 ,用酶免疫分析法 (EIA)检测胸腔积液CEA水平。根据最终诊断结果 ,6 5例患者分成 2组 :(1)非恶性胸腔积液组 :35例 ,(2 )恶性胸腔积液组 :30例。并将端粒酶活性检测结果与胸腔积液CEA测定结果进行比较。结果 非恶性胸腔积液中端粒酶阳性 2例 (5 7% ) ,恶性胸腔积液中阳性 2 7例 (90 % ) ,端粒酶活性测定诊断恶性胸腔积液的灵敏度为 0 90 ,特异度 0 94,阳性预测值 0 93 ,阴性预测值0 92 ,正确率 0 92。CEA诊断灵敏度为 0 6 0 ,特异度 0 89,阳性预测值 0 82 ,阴性预测值 0 72 ,正确率0 75。结论 胸腔积液端粒酶活性鉴别良恶性胸腔积液的诊断效能明显优于CEA测定 ,可作为一种诊断恶性胸腔积液的的辅助手段
Objective To study the differential diagnostic value of telomerase activity in benign and malignant pleural effusions and to compare with carcinoembryonic antigen (CEA). Methods The telomerase activity in pleural effusion was detected by polymerase chain reaction-enzyme-linked immunosorbent assay (PCR ELISA), and CEA level in pleural effusion was detected by enzyme immunoassay (EIA). According to the final diagnosis, 65 patients were divided into two groups: (1) non-malignant pleural effusion group: 35 cases, (2) malignant pleural effusion group: 30 cases. The result of telomerase activity was compared with CEA results of pleural effusion. Results The positive rate of telomerase in non-malignant pleural effusion was 2 (57%), in malignant pleural effusion (27%, 90%). The sensitivity of telomerase activity in the diagnosis of malignant pleural effusion was 90% Degree 0 94, positive predictive value 0 93, negative predictive value 0 92, correct rate 0 92. The diagnostic sensitivity of CEA was 0 60, the specificity was 0 89, the positive predictive value was 0 82, the negative predictive value was 0 72, and the correct rate was 0 75. Conclusion The diagnostic efficacy of pleural effusion telomerase activity in differentiating benign and malignant pleural effusions is significantly better than that of CEA, which can be used as an adjunct to diagnose malignant pleural effusions