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目的研究铅对五指山猪(WZSP)肝脏的毒性作用,为研究铅对人类肝脏损伤的风险评估提供理论参考。方法将20头健康3月龄清洁级WZSP随机分为5组,每组4头(雌雄各半,雄性去势),用含铅量分别为0.046、6.790、17.370、53.600和154.000mg/kg的饲料对其进行饲喂染毒,连续100d。观察肝脏表观特征的变化,并测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)和γ-谷氨酰基转移酶(γ-GGT)的活力。结果随着染铅剂量的增高,ALT活力呈现明显的上升趋势,差异均有统计学意义(P<0.05或P<0.01);AST活力也呈现递增的趋势,但与对照组相比,差异无统计学意义(P>0.05);γ-GGT活力逐渐升高,154.000mg/kg染铅组γ-GGT活力高于对照组,差异有统计学意义(P<0.05);ALP活力与对照组相比,差异均无统计学意义(P>0.05)。154.000mg/kg染铅组的肝脏表观特征发生变化,出现颜色变暗,包膜与实体脱离及黄疸症状。结论铅暴露可对WZSP肝脏产生毒性作用。
Objective To study the toxic effect of lead on the liver of WZSP pigs and to provide a theoretical reference for studying the risk assessment of lead on human liver injury. Methods Twenty healthy 3-month-old clean WZSPs were randomly divided into 5 groups with 4 rats in each group (male and female, castration), with lead contents of 0.046, 6.790, 17.370, 53.600 and 154.000 mg / kg Feeds for feeding contaminated, continuous 100d. The changes of liver appearance were observed, and the changes of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and γ-glutamyltransferase (γ -GGT) activity. Results The ALT activity increased significantly with the increase of lead exposure dose (P <0.05 or P <0.01), and the activity of AST also showed an increasing trend. However, compared with the control group, the difference was not significant (P> 0.05). The activity of γ-GGT increased gradually. The activity of γ-GGT in 154.000mg / kg lead group was higher than that of control group (P <0.05) The differences were not statistically significant (P> 0.05). The apparent characteristics of liver in 154.000 mg / kg lead-exposed group changed, darkening, capsule-entity separation and jaundice symptoms appeared. Conclusion Lead exposure may produce toxic effects on WZSP liver.